A Simplified Technique for the Agar Cup Assay of Penicillin.
نویسنده
چکیده
منابع مشابه
Methods of Measuring Penicillin Concentrations in Body Fluids.
Inhibition of bacterial growth is the basis of the three methods commonly used for determining the potency of penicillin solutions. With the Oxford cup method (Abraham et al., 1941; Foster and Woodruff, 1944), penicillin is allowed to diffuse out into agar plates from small glass cups, and the diameters of the zones of inhibition of bacterial growth are measured. In dilution methods (Foster and...
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The penicillin allergy is being increasingly recognized as a significant public health problem. Immunological responses to penicillin and other beta-lactam antibiotics are classified as immediate and non-immediate responses. This research aimed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of the reactive antibody value against penicillin in various species of animals...
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Official Circular 198 (Ruehle and Brewer, 1931) gives directions for performing the agar cup-plate test. However, in the limited literature dealing with the subject, no mention is made of the influence of varying the volume of agar, the amount of inoculum, the hydrogen ion concentration or other factors. In the course of other studies with the agar cup-plate method (Rose and Miller, 1939; Mille...
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Susceptibility disks impregnated with 35 different antibiotics were assayed by the current Code of Federal Regulations method and by a new method employing an agar overlay. The new method, a simplified analytical procedure, exhibited improvement in zone definition and an increase in dose response. Assays performed by the new method were accurate and precise. The agar overlay technique appears t...
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An agar diffusion method using paper disks on plates seeded with Clostridium perfringens and incubated anaerobically to assay serum levels of metronidazole is described. The lowest serum metronidazole concentration that could be measured was 0.7 mug/ml, and the average error was 4% for 5 mug/ml. This assay could measure metronidazole in the presence of gentamicin or in the presence of penicilli...
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عنوان ژورنال:
- Journal of bacteriology
دوره 47 4 شماره
صفحات -
تاریخ انتشار 1944